Membrane:Article Title: Optimized New Shengmai Powder suppresses ferroptosis in ischemic cardiomyocytes via cGMP-PKG signalling
Article Snippet: .. The PVDF membrane was then blocked at room temperature for 30 min with rapid blocking solution (P0252-100 mL, Bioyuntian, Shanghai, China) and incubated with primary antibodies (AKT1: bsm-52010R, p-AKT: bs-10996R, eNOS: bs-20609R, STAT3: bsm-52235R, p-STAT3: bsm-52210R, p-PKG1: bs-7368R, ACTB: bs-0061R, VCL: bsm-54148R, BIOSS, Beijing, China; p-eNOS: AF3247, sGC: AF0619, GPX4: DF6701, Affinity, Liyang, Jiangsu, China; PKG1: A01708-3, Boster, Wuhan, Hubei, China) at 4 °C overnight (12 h). ..
Article Title: Optimized New Shengmai Powder suppresses ferroptosis in ischemic cardiomyocytes via cGMP-PKG signalling.
Article Snippet: .. The PVDF membrane was then blocked at room temperature for 30 min with rapid blocking solution (P0252-100 mL, Bioyuntian, Shanghai, China) and incubated with primary antibodies (AKT1: bsm-52010R, p-AKT: bs-10996R, eNOS: bs-20609R, STAT3: bsm-52235R, p-STAT3: bsm-52210R, p-PKG1: bs-7368R, ACTB: bs-0061R, VCL: bsm-54148R, BIOSS, Beijing, China; p-eNOS: AF3247, sGC: AF0619, GPX4: DF6701, Affinity, Liyang, Jiangsu, China; PKG1: A017083, Boster, Wuhan, Hubei, China) at 4 °C overnight (12 h). .. After three washes with 1 × TBST, HRP-conjugated secondary antibodies were applied at room temperature for 1 h. Following additional washes, an ECL chemiluminescent substrate was added, and target protein bands were visualized using a chemiluminescence imaging system (ChemiScope 6100, Qinxian Scientific Instruments Co., Ltd., Shanghai, China). qRT‐PCR Total RNA was extracted from H9C2 cells using a total RNA extraction kit (G3640-50 T, Servicebio, Wuhan, Hubei, China).
Blocking Assay:Article Title: Optimized New Shengmai Powder suppresses ferroptosis in ischemic cardiomyocytes via cGMP-PKG signalling
Article Snippet: .. The PVDF membrane was then blocked at room temperature for 30 min with rapid blocking solution (P0252-100 mL, Bioyuntian, Shanghai, China) and incubated with primary antibodies (AKT1: bsm-52010R, p-AKT: bs-10996R, eNOS: bs-20609R, STAT3: bsm-52235R, p-STAT3: bsm-52210R, p-PKG1: bs-7368R, ACTB: bs-0061R, VCL: bsm-54148R, BIOSS, Beijing, China; p-eNOS: AF3247, sGC: AF0619, GPX4: DF6701, Affinity, Liyang, Jiangsu, China; PKG1: A01708-3, Boster, Wuhan, Hubei, China) at 4 °C overnight (12 h). ..
Article Title: Optimized New Shengmai Powder suppresses ferroptosis in ischemic cardiomyocytes via cGMP-PKG signalling.
Article Snippet: .. The PVDF membrane was then blocked at room temperature for 30 min with rapid blocking solution (P0252-100 mL, Bioyuntian, Shanghai, China) and incubated with primary antibodies (AKT1: bsm-52010R, p-AKT: bs-10996R, eNOS: bs-20609R, STAT3: bsm-52235R, p-STAT3: bsm-52210R, p-PKG1: bs-7368R, ACTB: bs-0061R, VCL: bsm-54148R, BIOSS, Beijing, China; p-eNOS: AF3247, sGC: AF0619, GPX4: DF6701, Affinity, Liyang, Jiangsu, China; PKG1: A017083, Boster, Wuhan, Hubei, China) at 4 °C overnight (12 h). .. After three washes with 1 × TBST, HRP-conjugated secondary antibodies were applied at room temperature for 1 h. Following additional washes, an ECL chemiluminescent substrate was added, and target protein bands were visualized using a chemiluminescence imaging system (ChemiScope 6100, Qinxian Scientific Instruments Co., Ltd., Shanghai, China). qRT‐PCR Total RNA was extracted from H9C2 cells using a total RNA extraction kit (G3640-50 T, Servicebio, Wuhan, Hubei, China).
Incubation:Article Title: Optimized New Shengmai Powder suppresses ferroptosis in ischemic cardiomyocytes via cGMP-PKG signalling
Article Snippet: .. The PVDF membrane was then blocked at room temperature for 30 min with rapid blocking solution (P0252-100 mL, Bioyuntian, Shanghai, China) and incubated with primary antibodies (AKT1: bsm-52010R, p-AKT: bs-10996R, eNOS: bs-20609R, STAT3: bsm-52235R, p-STAT3: bsm-52210R, p-PKG1: bs-7368R, ACTB: bs-0061R, VCL: bsm-54148R, BIOSS, Beijing, China; p-eNOS: AF3247, sGC: AF0619, GPX4: DF6701, Affinity, Liyang, Jiangsu, China; PKG1: A01708-3, Boster, Wuhan, Hubei, China) at 4 °C overnight (12 h). ..
Article Title: Optimized New Shengmai Powder suppresses ferroptosis in ischemic cardiomyocytes via cGMP-PKG signalling.
Article Snippet: .. The PVDF membrane was then blocked at room temperature for 30 min with rapid blocking solution (P0252-100 mL, Bioyuntian, Shanghai, China) and incubated with primary antibodies (AKT1: bsm-52010R, p-AKT: bs-10996R, eNOS: bs-20609R, STAT3: bsm-52235R, p-STAT3: bsm-52210R, p-PKG1: bs-7368R, ACTB: bs-0061R, VCL: bsm-54148R, BIOSS, Beijing, China; p-eNOS: AF3247, sGC: AF0619, GPX4: DF6701, Affinity, Liyang, Jiangsu, China; PKG1: A017083, Boster, Wuhan, Hubei, China) at 4 °C overnight (12 h). .. After three washes with 1 × TBST, HRP-conjugated secondary antibodies were applied at room temperature for 1 h. Following additional washes, an ECL chemiluminescent substrate was added, and target protein bands were visualized using a chemiluminescence imaging system (ChemiScope 6100, Qinxian Scientific Instruments Co., Ltd., Shanghai, China). qRT‐PCR Total RNA was extracted from H9C2 cells using a total RNA extraction kit (G3640-50 T, Servicebio, Wuhan, Hubei, China).
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